mouse rat fgf23 intact elisa kit  (Quidel)

Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: Distinct roles of ferric carboxymaltose and ferric derisomaltose on phosphate homeostasis in iron deficiency anemia

doi: 10.1016/j.ejps.2025.107265

Figure Lengend Snippet: After induction of IDA, (A) hematocrit, (B) number of red blood cells, (C) hemoglobin, and (D) MCV significantly decreased. (E) The number of white blood cells was unaffected, but (F) thrombocyte numbers significantly increased. (G) Plasma P i significantly increased, but (H) plasma Ca 2+ was unaffected. (I) PTH also decreased in response to induction of IDA, (J) no effect was observed on intact FGF23, but (K) C-terminal FGF23 significantly increased. Consequently, there was a significant reduction in the intact:C-terminal FGF23 ratio (L). In addition to individual data, mean ± S .E.M. are shown. Data were analyzed by a paired Student’s t -test. n = 40 female mice. # P < 0.05 versus baseline.

Article Snippet: Plasma hormones were measured using enzyme-linked immunosorbent assays (ELISA) for PTH (cat. #60–2305, Quidel, San Diego, CA), intact FGF23 (cat. #60–6800, Quidel) and C-terminal FGF23 (cat. #60–6300, Quidel) ( ).

Techniques: Clinical Proteomics

Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: Distinct roles of ferric carboxymaltose and ferric derisomaltose on phosphate homeostasis in iron deficiency anemia

doi: 10.1016/j.ejps.2025.107265

Figure Lengend Snippet: (A) At the end of the experimental period, PTH was significantly lower in vehicle-treated anemic mice, whereas FCM and FDI treatment did not affect PTH levels. (B) Intact FGF23 significantly increased in vehicle-treated mice, but no effect was seen in FCM- and FDI-treated mice. (C) C-terminal FGF23 remained unchanged in vehicle-treated mice, while FCM- and FDI-treated mice had significantly reduced C-terminal FGF23. (D) The ratio of intact:C-terminal FGF23 increased independently of treatment but was highest in FDI-treated mice. There is an inverse correlation between plasma iron levels and (E) intact FGF23 levels and (F) C-terminal FGF23 levels. Data are expressed as mean ± S .E.M. and were analyzed by a repeated measures mixed-effects model followed by Tukey’s multiple comparisons test. n = 12–16 female mice/group. * P < 0.05 versus vehicle, # P < 0.05 versus baseline, § P < 0.05 versus FCM.

Article Snippet: Plasma hormones were measured using enzyme-linked immunosorbent assays (ELISA) for PTH (cat. #60–2305, Quidel, San Diego, CA), intact FGF23 (cat. #60–6800, Quidel) and C-terminal FGF23 (cat. #60–6300, Quidel) ( ).

Techniques: Clinical Proteomics

Journal: Cell Biochemistry and Biophysics

Article Title: Chorein Regulates Key Osteoblast Genes in UMR-106 Cells

doi: 10.1007/s12013-025-01939-4

Figure Lengend Snippet: Effect of CRISPR/Cas9-induced Vps13a knockdown on FGF23 production. ( A ) Fgf23 mRNA ( n = 7) expression normalized to β-actin ( Actb ) in control cells transfected with non-targeting (NT) sequence and Vps13a knockdown (KD) cells using CRISPR/Cas9 system. ( B ) C-terminal FGF23 protein concentration ( n = 8) in the cell culture supernatant of NT and Vps13a knockdown cells. All data are shown as arithmetic means ± SEM. (A: Unpaired Student’s t -test; B: Mann-Whitney U test)

Article Snippet: The concentration of FGF23 protein in the cell culture supernatant was determined using ELISA kits (mouse/rat FGF23 (C-Term) and mouse/rat FGF23 (intact), both from Quidel, Cologne, Germany).

Techniques: CRISPR, Knockdown, Expressing, Control, Transfection, Sequencing, Protein Concentration, Cell Culture, MANN-WHITNEY

Journal: Cell Biochemistry and Biophysics

Article Title: Chorein Regulates Key Osteoblast Genes in UMR-106 Cells

doi: 10.1007/s12013-025-01939-4

Figure Lengend Snippet: Knockdown of Vps13a influences mRNA expression of FGF23 regulators . Relative mRNA expression of Phex ( A , n = 6) and Galnt3 ( B , n = 8) mRNA normalized to β-actin ( Actb ) in non-targeting (NT) control cells and Vps13a knockdown (KD) cells. All data are shown as arithmetic means ± SEM. (A: Unpaired t -test with Welch’s correction; B: Unpaired Student’s t -test)

Article Snippet: The concentration of FGF23 protein in the cell culture supernatant was determined using ELISA kits (mouse/rat FGF23 (C-Term) and mouse/rat FGF23 (intact), both from Quidel, Cologne, Germany).

Techniques: Knockdown, Expressing, Control

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is down-regulated by pTxA2 in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM of rel. Fgf23 gene expression in UMR-106 ( a , n = 5) and MC3T3-E1 ( b , n = 5) cells treated without or with pTxA2 for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is decreased by TxA2 agonist I-BOP in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM of rel. Fgf23 gene expression in UMR-106 ( a , n = 6) and in MC3T3-E1 ( b , n = 7) cells treated without or with TxA2 agonist I-BOP for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is inhibited by TxA2 agonist U46619 in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM ( n = 5) of rel. Fgf23 gene expression in UMR-106 ( a ) and MC3T3-E1 ( b ) cells treated without or with TxA2 agonist U46619 for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: C-terminal FGF23 concentration in the supernatant of UMR-106 cells is lowered by TxA2 agonists. Arithmetic means ± SEM of C-terminal FGF23 concentration in the supernatant of UMR-106 cells treated without or with 100 ng/mL pTxA2 ( a , n = 7), 100 ng/mL I-BOP ( b , n = 7) or 5 µ m U46619 ( c , n = 8) for 24 h. * p < 0.05, ** p < 0.01. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Concentration Assay

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: TxA2 antagonist SQ29548 reverses the effect of I-BOP on Fgf23 mRNA transcripts. Arithmetic means ± SEM ( n = 10) of relative Fgf23 gene expression in UMR-106 cells treated without or with 100 ng/mL TxA2 agonist I-BOP in the presence or absence of 1 µ m TxA2 antagonist SQ29548 for 24 h. ** p < 0.01. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Exposure to activated platelets suppresses Fgf23 mRNA transcripts in UMR-106 cells. Arithmetic means ± SEM ( n = 8) of relative Fgf23 gene expression in UMR-106 cells exposed or not exposed to 100,000/µL freshly isolated human platelets in the presence or absence of 1 U/mL thrombin. * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Gene Expression, Isolation

Journal: bioRxiv

Article Title: Kidney cortex macrophages prevent the formation of mineral stones by reabsorbing phosphate from the urine

doi: 10.1101/2025.02.18.638798

Figure Lengend Snippet: ( A ) Scheme illustrating the generation of Slc34a1 ΔMØ mice and the experimental protocol of Panels B-E in which Slc34a1 ΔMØ mic were treated with either corn oil (Oil) or tamoxifen (TAM). ( B ) Efficiency of Slc34a1 abrogation in kidney cortex MØ, examined by RT-PCR. Each dot represents a pool of cells derived from 3 mice. ( C ) Urine output of the indicated electrolytes, adjusted by the excreted creatinine, before and after TAM treatment. ( D ) The concentrations of PTH and FGF23 in the blood plasma. ( E ) The relative expression of Cyp27b1 and Cyp24a1 transcripts in the kidney. ( F ) Scheme illustrating the protocol of low-phosphorus diet experiments for Panels G-I. ( G and H ) Urine Pi output (G) and Pi concentrations in blood plasma (H). ( I ) In open-field test, after the indicated treatments, Slc34a1 ΔMØ mice were placed in a square arena, and their total moving distance (left) and immobile time (right) were recorded during the observation period. ( J ) In rotarod test, after the indicated treatments, Slc34a1 ΔMØ mice were placed on a rod which then started to rotate in an accelerated speed. The on-rod time periods before their falling down were recorded. ns, not significant. * P <0.05, ** P <0.01, **** P <0.001 by two-tailed unpaired t test (B, D, E, I, J), two-tailed paired t test (C) and two-way ANOVA (G, H). Data are depicted as mean±SEM. Data are derived from at least 2 independent experiments.

Article Snippet: Plasma levels of PTH and FGF23 were measured by ELISA kits (Elabscience, #E-EL-M0709; Elabscience, #E-EL-M2415, respectively), following the manufacturer’s instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Expressing, Two Tailed Test